Skip to main content

How to perform efficient Pipetting with difficult liquids


In most labs where pipetting operations are regularly conducted, certain analysis and operations involve challenging liquids. These liquids may be viscous, light-sensitive, or possess fluorescent properties. It is also likely that the operations may involve handling of fluids under strict conditions or be corrosive or dangerous if it meets skin or other surfaces.

In such cases, pipetting becomes a challenge. Nevertheless, it is possible with due care and proper preparation.


Tips to pipette fluids of varying density and samples that require high precision results

If the density of a liquid is very low, as in the case of PCR reagents, pipetting can be performed efficiently with narrow orifice tips. Along with an extended tip support, the addition of an extra narrow tip will improve the reproducibility of the results. Standard tips of 10uL will work fine with liquids that have densities similar to that of water.

In certain lab operations, high precision and reproducibility of results becomes essential. In these cases, top accuracy must be maintained. Low retention tips become essential for such operations, as these allow maximum fluid dissipation. Low retention tips usually contain plastic polymers inside the tips, which encourages a smooth flow. The same tips can also be used when working with sensitive or expensive fluids in operations that require minimum wastage or retention of samples.

Pipetting tips for samples under strict aseptic conditions and light-sensitive substances

Individually wrapped tips are highly recommended for operations that involve samples under strict aseptic conditions. Sterile pipette tips such as these are less prone to contamination and will ensure sterility of the sample during the pipetting phase. This will also improve the performance level and make sure the results are accurate.

Certain experiments and lab operations must be conducted on fluorescent and light sensitive substances. In such cases, it is important to take due care during pipetting phase to ensure accurate results. The results should also be perfectly reproducible for repeated operations in the same operating conditions. For light-sensitive samples, protection of the sample from light can be ensured by wrapping the samples in aluminum foil to protect it from the external light rays.

Black microcentrifuge tubes are also a practical choice when the operation involves photo-sensitive or fluorescent substances. These tubes are different from the typical amber tinted tubes, since they are completely opaque. Hence, they will not interfere with the light-sensitive samples and enable them to retain their original integrity during the process.

Contact FirstSource Laboratory Solutions if you are looking for Fluid Handling instruments, Analytical, Filtration, Sterilization or any other lab setup equipment’s.
Labels:

Comments

Post a Comment

Popular posts from this blog

Features Of Analytical Balances That Can Be Used For Research Purposes

A scientific research environment needs absolute precision.   These research activities require absolute and accurate measurements of different factors such as temperature, timing and weight. The use of modern technology in manufacturing various testers and measurement devices have led to authentic products like analytical weighing scales, digital thermometers and other measuring instruments that give precise and absolute readings. Analytical balances are the sought-after devices for scientific research purposes. These balances are very accurate and show readings up to seven decimal places. Such accurate readings are very essential for success of an experiment. Along with being accurate, analytical balances are also functional. These balances are able to read the weight of the samples and show minor dispersions on the scale platforms as well. The most important feature of RADWAG analytical balance is its zeroing capability. An ideal balance should take less than five s...
Post a Comment
Read more

How to know whether your UV/VIS Verification methods conform to USP 857 Standards

The United States Pharmacopeial Convention 857 or USP 857 necessitates that all equipment imported into the USA by pharmaceutical companies in the country should mandatorily satisfy their set of requirements. This mandate was established and brought into play on 1st May 2016 and continues to affect all vendors in this field of operation. The USP 857 standards contain very specific criteria for verification of wavelength accuracy and photometric accuracy of concerned equipments. There are also new requirements suggested for spectral resolutions and stray light which wasn't present previously. Apart from this, they have also laid out very specific requirements for filters for each verification that is conducted. These USP requirements are more in tandem with International Pharmacopeial requirements. All new and existing laboratories in the USA are expected to conform to these new set standards and actively involve in testing these changes which are now recognized officially. ...
Post a Comment
Read more

3 Easy Steps for Safe Solvent Extraction

In the botanical industry, there are many options available for extracting soluble components of plants. However solvent extraction is the easiest, most inexpensive and fastest method. Solvent Extraction as the name says is extraction of essential oils from plants by using a solvent. Solvent extraction is also considered to be the safest forms of extraction process and provide quite similar results compared to other options available. This process is used on plants that cannot resist heat and thereby making it easy to capture their fragrances. Steps to follow for a safe, easy and fastest extraction process: Ethanol is a one of the widely used and most popular high-proof alcohol used for solvent extraction. It is safe to use it for infused edibles and at the same time can be used with any type of container. Ethanol is well-known to provide uniform results and is easily recoverable from the substance. Step 1: Soak   Soak the plant material completely in the et...
Post a Comment
Read more